Focus On: Oligonucleotides, Radiolabelling and Metabolism
The design and application of oligonucleotides as therapeutic agents require a basic understanding of their pharmacokinetic properties and bio-distribution. Radiolabelled tracers play an essential role in developing such an understanding. Pharmaron is the largest provider of custom radiosynthesis services using 14C and tritium and can synthesize radiolabelled oligonucleotides using traditional and automated synthetic methods.
Pharmaron can assess ADME properties of radiolabelled oligonucleotides by conducting pharmacokinetic, excretion balance, and biodistribution studies, including quantitative whole body autoradiography (QWBA) and micro autoradiography (MARG), and has recently demonstrated that high-quality pharmacokinetic and tissue distribution data can be obtained following radiolabelling of oligonucleotides with tritium. The PK and tissue disposition of potent oligonucleotides radiolabelled with 14C can also be determined using AMS detection. Pharmaron (formerly Xceleron) developed a novel assay combining different methods of chromatography of separation and detection, SPE, UPLC, and AMS, to provide high-quality PK data for parent 14C-oligonucleotide in plasma, urine, and tissues such as liver and kidney.
Learn more about our radiolabeling services
