Tiny Tubes, Big Impact: Advancing 3R Principles by Developing Capillary Microsampling LC–MS/MS for the Quantification of CRN04894 in Rat Plasma

Poster on capillary microsampling LC–MS/MS for CRN04894 quantification, featuring global and molecular visuals with "Tiny Tubes, Big Impact" title.

Poster Authors:

Mitesh Sanghvi1, Junlong Shao1, Angel Madariaga1, Stephanie Dunchock2, Jian Wang2 and Barry Jones2

1Pharmaron, 20340 Seneca Meadows Parkway, Germantown, MD, 20876, USA

2Crinetics Pharmaceuticals, 6055 Lusk Blvd., San Diego, CA 92121, USA

Capillary Microsampling LC–MS/MS

Incorporating capillary microsampling (CMS) in preclinical pharmacokinetic (PK) studies aligns with the 3R principles (Replacement, Reduction, Refinement) and reduces animal stress induced by invasive techniques like venipuncture. However, CMS requires a more sensitive LC-MS/MS method for reliable analysis.

In this study, we aim to develop, qualify, and apply a sensitive LC–MS/MS method for quantifying the small molecule CRN04894 in rat plasma using CMS, with the goal of characterizing its PK profile.