Poster Authors:

Wendy Cheung¹, Nigus Ambaye¹

¹Pharmaron, 20340, Seneca Meadows Parkway, Germantown, MD, 20876, USA

Development of a Hybridization ELISA Assay

Hybridization ELISA is a highly sensitive method used to quantify antisense oligonucleotides (ASO) via hybridization of a probe with a specific sequence. The method can detect low concentrations of these molecules in complex biological matrices like serum. Recent advancements of the technology have positioned it as a robust alternative to mass spectrometry, LC-MS, and qPCR.

In this poster, we present the optimization of key parameters of this method to enhance stability and sensitivity by modifying probe capture, detection methods, and hybridization conditions. The MSD readout demonstrated a superior dynamic range in detecting the targeted siRNA compared to the fluorescent ELISA readout.