Upstream AAV Production: A Targeted Approach to Optimization
Recorded: February 2022
This webinar discusses key upstream process parameters to consider for efficient and scalable AAV production with a focus on the transfection step.
This webinar will discuss some of the key upstream process parameters to consider for efficient and scalable AAV production with a focus on the transfection step.
Key messages
- What are the critical upstream parameters for AAV production??
- What early process considerations are required to ensure success at large scale??
- What high-throughput technologies can accelerate development timelines and deliver a robust platform??
- How can innovation drive a fast-changing landscape?
Agenda
- What AAV attributes are important to ensure high-yield and high-quality AAV production?
- Performance and quality attributes: titre, infectivity, E:F, impurities?
- Cell line/Media importance?
- Transfection as a critical step: show different approaches are possible and why chemical based is the method of choice?
- Improvement of AAV production phase: mechanisms to improve titres, lysis vs non lysis and serotype impact.
- ?What is Pharmaron’s current approach? ?
- Platform process and feasibility studies to assess fit (including impact to DSP)?
- Use of high-throughput technology in combination with DoE to optimise key parameters?
- Importance of scale-up with a focus on transfection?
- Pharmaron’s AAV USP platform performance (yield, impurities, infectivity data)?
- Introduce Polyplus and importance of collaboration?
- Collaboration and trial of transfection reagent?
- Introduction to Polyplus and their products?
- Presentation of Pharmaron Beta testing data?
- Presentation of internal Pharmaron data using Polyplus product